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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which ends up in chromosome instability, and so might contribute to tumorigenesis. This protein was found to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant function in endorsing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two supplemental splice variants have been reported but detailed nucleotide sequence facts is just not but accessible.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...
Antibodies bound to the blocking peptide now not bind to your epitope to the goal protein. By evaluating the staining with the blocked antibody compared to the antibody alone, you can see which staining is particular.
Concentrate on Details Cyclin E belongs for the remarkably conserved cyclin loved ones, whose users are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins check here show unique expression and degradation designs which contribute towards the temporal coordination of each mitotic event. Cyclin E forms a posh with and capabilities for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary which is degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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